SEURAT-1 partners Mathieu Vinken and Vera Rogiers, both affiliated to the Department of In Vitro Toxicology and Dermato-Cosmetology of the Vrije Universiteit Brussel, have recently edited a book entitled ‘Protocols in In Vitro Hepatocyte Research’ published by Springer (ISBN 978-1-4939-2073-0). This book presents 30 state-of-the-art protocols and reviews to set up and apply primary hepatocyte cultures for research and screening purposes. The first part of the book focuses on the use of these particular liver-based in vitro models to study the different aspects of the hepatocyte life cycle, including cell growth, differentiation and cell death. The second part of the book is targeted towards the demonstration of the applicability of primary hepatocyte cultures, or liver-based in vitro models derived thereof, for functionality and toxicity testing. Written in the highly successful ‘Methods in Molecular Biology’ series format (volume 1250), chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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